Journal: Oncology Reports

Article Title: Image analysis of the nuclear characteristics of emerin protein and the correlation with nuclear grooves and intranuclear cytoplasmic inclusions in lung adenocarcinoma

doi: 10.3892/or.2018.6848

Figure Lengend Snippet: Flow chart of the analysis in this study shows the Feulgen reaction and emerin IHC. IHC, immunohistochemistry.

Article Snippet: After the solution was removed by tapping, the specimens were treated with mouse monoclonal anti-human emerin antibody (1:500 dilution, clone CL0201; cat. no. NBP2-52876; Novus Biologicals, Littleton, CO, USA) at 4°C overnight.

Techniques: Immunohistochemistry

Journal: Oncology Reports

Article Title: Image analysis of the nuclear characteristics of emerin protein and the correlation with nuclear grooves and intranuclear cytoplasmic inclusions in lung adenocarcinoma

doi: 10.3892/or.2018.6848

Figure Lengend Snippet: (A-D) Representative effect of specimen thickness in the nuclear analysis of emerin-stained specimens after image analysis and (E-G) representative data of the correlation analysis for nuclear size, NP or ENML for specimens stained by immunohistochemistry (IHC) for emerin. Effect of section thickness is shown in case no. 14: (A and B) 1-µm-thick, (C and D) 2-µm-thick. NP as detected by our protocol is indicated in red, while the emerin-stained membrane-traced line is indicated in green (B and D). (E-G) Correlation of nuclear area (Avg), NP (Avg), and ENML. Emerin in the x-axis in (G) stands for ENML (emerin-stained nuclear membrane length). Images in A-D were captured using VS (×40 objective lens). NP, nuclear perimeter; VS, virtual slide scanner.

Article Snippet: After the solution was removed by tapping, the specimens were treated with mouse monoclonal anti-human emerin antibody (1:500 dilution, clone CL0201; cat. no. NBP2-52876; Novus Biologicals, Littleton, CO, USA) at 4°C overnight.

Techniques: Staining, Immunohistochemistry, Membrane

Journal: Oncology Reports

Article Title: Image analysis of the nuclear characteristics of emerin protein and the correlation with nuclear grooves and intranuclear cytoplasmic inclusions in lung adenocarcinoma

doi: 10.3892/or.2018.6848

Figure Lengend Snippet: A representative case with predominantly smooth nuclei (case no. 24) (A and C) and a case with eccentric nuclei (case no. 97) (B and D) as shown by emerin immunohistochemistry (IHC). In B and D, the NP is indicated in red and the ENML traced by the software is shown in green. Avg data of NP and ENML (emerin) and NP minus ENML is shown at the bottom of the figure. All images were captured by a VS (×40 objective lens). NP, nuclear perimeter; ENML, emerin-stained nuclear membrane length; VS, virtual slide scanner.

Article Snippet: After the solution was removed by tapping, the specimens were treated with mouse monoclonal anti-human emerin antibody (1:500 dilution, clone CL0201; cat. no. NBP2-52876; Novus Biologicals, Littleton, CO, USA) at 4°C overnight.

Techniques: Immunohistochemistry, Software, Staining, Membrane

Journal: Oncology Reports

Article Title: Image analysis of the nuclear characteristics of emerin protein and the correlation with nuclear grooves and intranuclear cytoplasmic inclusions in lung adenocarcinoma

doi: 10.3892/or.2018.6848

Figure Lengend Snippet: (A and C) Representative nuclear groove and intracytoplasmic inclusion, and (B and D) the relationship among nuclear grooves and intracytoplasmic inclusions, and a high nuclear deformity rate among all nuclei (NP minus the ENML-negative nuclei ratio out of all nuclei) in emerin-stained specimens. (E) The correlation between nuclear area and high nuclear deformity rate (HNDR) out of all nuclei. (A and C) A representative nuclear groove is seen as a ‘coffee bean shape’, indicated by arrows in A. A representative intracytoplasmic inclusion is indicated by an arrow in C. On the left is an H&E-stained section, whereas the right panel shows emerin IHC (×100 objective lens). (B and D) On the x-axis, a high nuclear deformity rate out of all nuclei was categorized for every 5%. The total number of cases in each category is shown in the gray bar. The percentage of cases with nuclear grooves (NGs) (B) or intranuclear cytoplasmic inclusions (ICIs) (D) out of all cases in each category is shown in the line plot. (E) Correlation between nuclear area and the rate of HNDN. NP, nuclear perimeter; ENML, emerin-stained nuclear membrane length.

Article Snippet: After the solution was removed by tapping, the specimens were treated with mouse monoclonal anti-human emerin antibody (1:500 dilution, clone CL0201; cat. no. NBP2-52876; Novus Biologicals, Littleton, CO, USA) at 4°C overnight.

Techniques: Staining, Membrane

Journal: Oncology Reports

Article Title: Image analysis of the nuclear characteristics of emerin protein and the correlation with nuclear grooves and intranuclear cytoplasmic inclusions in lung adenocarcinoma

doi: 10.3892/or.2018.6848

Figure Lengend Snippet: Comparison of cases with low emerin expression (n=34) and cases with positive emerin expression (n=54). (A) Nuclear area, (B) NP, (C) shape factor, and (D) staining intensity of each group were analyzed by Student's t-test. NP, nuclear perimeter.

Article Snippet: After the solution was removed by tapping, the specimens were treated with mouse monoclonal anti-human emerin antibody (1:500 dilution, clone CL0201; cat. no. NBP2-52876; Novus Biologicals, Littleton, CO, USA) at 4°C overnight.

Techniques: Comparison, Expressing, Staining

Journal: Cell reports

Article Title: Synthetic Lethal Interaction between the ESCRT Paralog Enzymes VPS4A and VPS4B in Cancers Harboring Loss of Chromosome 18q or 16q

doi: 10.1016/j.celrep.2020.108493

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Emerin (Mouse monoclonal) , Novus Biologicals , NBP2-52877.

Techniques: Virus, Recombinant, CRISPR, Mouse Assay, Luciferase, Software, Simple Western, Quantitative Proteomics

Journal: World Journal of Surgical Oncology

Article Title: Collective analysis of the expression and prognosis for LEM-domain proteins in prostate cancer

doi: 10.1186/s12957-022-02640-z

Figure Lengend Snippet: The expressions of ANKLE1, EMD, and LEMD2 and their relationship with clinical parameters of PRAD. A – C ANKLE1, EMD, and LEMD2 levels were increased in prostate cancer tissues compared to normal tissues (RNA-seq data from TCGA PRAD). The number of the normal group is 52, and the number of the tumor group is 499. D – G Higher ANKLE1 expression was associated with age, N stage, T stage, and M stage. H – K Higher EMD expression was associated with age, N stage, T stage, and M stage. L – O Higher LEMD2 expression was associated with age, N stage, T stage, and M stage. Compared with indicated group, * p < 0.05, ** p < 0.01, *** p < 0.001. n.s., no significant difference

Article Snippet: After blocking with 5% non-fat milk, the membranes were probed with primary Abs ANKLE1 (Proteintech, 24080-1-AP, 1:1000), EMD (Proteintech, 10351-1-AP, 1:1000), and LEMD2 (Boster, M00714, 1:6000) overnight at 4 °C and then incubated with HRP-conjugated secondary Abs (1:7000) at room temperature for 2 h. The protein bands were examined by Fusion Fx7 (VILBER LOURMAT).

Techniques: RNA Sequencing, Expressing

Journal: World Journal of Surgical Oncology

Article Title: Collective analysis of the expression and prognosis for LEM-domain proteins in prostate cancer

doi: 10.1186/s12957-022-02640-z

Figure Lengend Snippet: The prognostic analysis of ANKLE1, EMD, and LEMD2 in prostate cancer. A – C The progress-free interval of ANKLE1, EMD, and LEMD2 mRNA level in prostate cancer patients (Kaplan-Meier plotter, tumor samples: n =499). D – F The overall survival of ANKLE1, EMD, and LEMD2 mRNA level in prostate cancer patients (Kaplan-Meier plotter, tumor samples: n = 499). G – L Time-dependent survival ROC curve analysis of ANKLE1, EMD, and LEMD2 to predict 3-, 5-, and 10-year survival rates

Article Snippet: After blocking with 5% non-fat milk, the membranes were probed with primary Abs ANKLE1 (Proteintech, 24080-1-AP, 1:1000), EMD (Proteintech, 10351-1-AP, 1:1000), and LEMD2 (Boster, M00714, 1:6000) overnight at 4 °C and then incubated with HRP-conjugated secondary Abs (1:7000) at room temperature for 2 h. The protein bands were examined by Fusion Fx7 (VILBER LOURMAT).

Techniques:

Journal: World Journal of Surgical Oncology

Article Title: Collective analysis of the expression and prognosis for LEM-domain proteins in prostate cancer

doi: 10.1186/s12957-022-02640-z

Figure Lengend Snippet: qPCR, WB, and IHC validation of ANKLE1, EMD, and LEMD2 in prostate cancer. A – C The mRNA levels of ANKLE1, EMD, and LEMD2 in human prostate tumor specimens were validated by qPCR. D – I The protein levels of ANKLE1, EMD, and LEMD2 in human prostate tumor specimens were validated by WB and IHC. The data (means ± SEM) shown ( A – C , n = 5; D – I , n = 3) were representative of three separate experiments. Compared with the indicated group, * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: After blocking with 5% non-fat milk, the membranes were probed with primary Abs ANKLE1 (Proteintech, 24080-1-AP, 1:1000), EMD (Proteintech, 10351-1-AP, 1:1000), and LEMD2 (Boster, M00714, 1:6000) overnight at 4 °C and then incubated with HRP-conjugated secondary Abs (1:7000) at room temperature for 2 h. The protein bands were examined by Fusion Fx7 (VILBER LOURMAT).

Techniques: Biomarker Discovery

Journal: World Journal of Surgical Oncology

Article Title: Collective analysis of the expression and prognosis for LEM-domain proteins in prostate cancer

doi: 10.1186/s12957-022-02640-z

Figure Lengend Snippet: The co‐expression genes with ANKLE1, EMD, and LEMD2 in prostate adenocarcinoma from the LinkedOmics database. A – C The significantly associated genes with ANKLE1, EMD, and LEMD2 were distinguished by the Pearson test in the prostate adenocarcinoma cohort. D – F KEGG pathways of ANKLE1, EMD, and LEMD2 in prostate adenocarcinoma cohort

Article Snippet: After blocking with 5% non-fat milk, the membranes were probed with primary Abs ANKLE1 (Proteintech, 24080-1-AP, 1:1000), EMD (Proteintech, 10351-1-AP, 1:1000), and LEMD2 (Boster, M00714, 1:6000) overnight at 4 °C and then incubated with HRP-conjugated secondary Abs (1:7000) at room temperature for 2 h. The protein bands were examined by Fusion Fx7 (VILBER LOURMAT).

Techniques: Expressing

Journal: World Journal of Surgical Oncology

Article Title: Collective analysis of the expression and prognosis for LEM-domain proteins in prostate cancer

doi: 10.1186/s12957-022-02640-z

Figure Lengend Snippet: A – C The Correlation between ANKLE1, EMD, and LEMD2 expressions and infiltrating immune cells in prostate cancer

Article Snippet: After blocking with 5% non-fat milk, the membranes were probed with primary Abs ANKLE1 (Proteintech, 24080-1-AP, 1:1000), EMD (Proteintech, 10351-1-AP, 1:1000), and LEMD2 (Boster, M00714, 1:6000) overnight at 4 °C and then incubated with HRP-conjugated secondary Abs (1:7000) at room temperature for 2 h. The protein bands were examined by Fusion Fx7 (VILBER LOURMAT).

Techniques:

Journal: World Journal of Surgical Oncology

Article Title: Collective analysis of the expression and prognosis for LEM-domain proteins in prostate cancer

doi: 10.1186/s12957-022-02640-z

Figure Lengend Snippet: Associations of ANKLE1, EMD, and LEMD2 expressions with immunomodulators and chemokines from the TISIDB database. A – I Correlations between immunomodulators (including immune inhibitors, immunostimulators, and MHC molecules) and the expression levels of ANKLE1, EMD, and LEMD2. J – L Correlations between chemokines and the expression levels of ANKLE1, EMD, and LEMD2

Article Snippet: After blocking with 5% non-fat milk, the membranes were probed with primary Abs ANKLE1 (Proteintech, 24080-1-AP, 1:1000), EMD (Proteintech, 10351-1-AP, 1:1000), and LEMD2 (Boster, M00714, 1:6000) overnight at 4 °C and then incubated with HRP-conjugated secondary Abs (1:7000) at room temperature for 2 h. The protein bands were examined by Fusion Fx7 (VILBER LOURMAT).

Techniques: Expressing

Journal: World Journal of Surgical Oncology

Article Title: Collective analysis of the expression and prognosis for LEM-domain proteins in prostate cancer

doi: 10.1186/s12957-022-02640-z

Figure Lengend Snippet: Comparison of KM survival curves of ANKLE1, EMD, and LEMD2 expressions based on immune cells. A High ANKLE1 levels enriched in resting NK cells had worse OS in PRAD. B High EMD levels enriched in resting NK cells had worse OS in PRAD. C High LEMD2 levels enriched in resting NK cells had worse OS in PRAD

Article Snippet: After blocking with 5% non-fat milk, the membranes were probed with primary Abs ANKLE1 (Proteintech, 24080-1-AP, 1:1000), EMD (Proteintech, 10351-1-AP, 1:1000), and LEMD2 (Boster, M00714, 1:6000) overnight at 4 °C and then incubated with HRP-conjugated secondary Abs (1:7000) at room temperature for 2 h. The protein bands were examined by Fusion Fx7 (VILBER LOURMAT).

Techniques: Comparison

Journal: World Journal of Surgical Oncology

Article Title: Collective analysis of the expression and prognosis for LEM-domain proteins in prostate cancer

doi: 10.1186/s12957-022-02640-z

Figure Lengend Snippet: The analysis of mutation, CNV, and methylation for ANKLE1, EMD, and LEMD2 expressions in PRAD. A – C Heat map showing the correlations between ANKLE1, EMD, and LEMD2 mRNA levels and somatic mutations, CNV, and methylation in prostate cancer through the UCSC Xena database

Article Snippet: After blocking with 5% non-fat milk, the membranes were probed with primary Abs ANKLE1 (Proteintech, 24080-1-AP, 1:1000), EMD (Proteintech, 10351-1-AP, 1:1000), and LEMD2 (Boster, M00714, 1:6000) overnight at 4 °C and then incubated with HRP-conjugated secondary Abs (1:7000) at room temperature for 2 h. The protein bands were examined by Fusion Fx7 (VILBER LOURMAT).

Techniques: Mutagenesis, Methylation